Xenobiotic biotransformation potential of Pseudomonas rhodesiae KCM-R5 and Bacillus subtilis KCM-RG5, tolerant to heavy metals and phenol derivatives

Abstract

Two environmental bacterial isolates KCM-R5 and KCM-RG5 were selected from xenobiotic-polluted environment. KCM-R5 was identified as Pseudomonas rhodesiae and KCM-RG5 as Bacillus subtilis. KCM-R5 demonstrated tolerance to heavy metals and KCM-RG5 to heavy metals and phenol derivatives. Both strains were studied for xenobiotic biotransformation
in order to contribute towards bioremediation of polluted environments.
Pseudomonas rhodesiae KCM-R5 and Bacillus subtilis. KCM-RG5 possess unusual ability to utilize ortho-nitrophenol (o-NP) and 2,4- dichlorophenoxyacetic acid (2,4-D). o-NP and 2,4-D were added at concentration 30 mg/l. The possible indictive/inhibiting effect of Pb cations (40mg/l) was also studied. Pseudomonas rhodesiae KCM-R5 removed 86 % of o-NP and below 1% of 2,4-D. Bacillus subtilis KCM-RG5 eliminated 83% of o-NP andunder 1% of 2,4-D. Biotransformation effectiveness of o-NP reached 95-100% in contrast to 2,4-D where the effectiveness was just 15-20%. Cell morphological changes were registered during the biotransformation processes. The obtained results could contribute to manage bioremediation processes in polluted with heavy metals and phenol derivatives environments.
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